mouse cxcl1 gro α  (MedChemExpress)

Journal: Zoological Research

Article Title: Elevated CXCL1 triggers dopaminergic neuronal loss in the substantia nigra of C57BL/6J mice: Evaluation of a novel Parkinsonian mouse model

doi: 10.24272/j.issn.2095-8137.2024.228

Figure Lengend Snippet: Linoleic acid (LA) and rasagiline (Rasa) inhibit CXCL1 elevation, movement disorders, and neuroinflammation induced by MPTP A: ELISA analysis of CXCL1 levels in the serum of mice in the MPTP-induced subacute model ( n =8, 10; P <0.05, Student’s t-test). B: ELISA analysis of CXCL1 levels in the serum of MPTP-induced chronic model mice pre-treated with LA and Rasa ( n =13, 15, 13, 14). C–F: Motor behavior assessment using the pole (C–E) and rotarod tests (F) in MPTP-treated mice with LA or Rasa pre-treatment ( n =13, 15, 13, 14). G–J: Immunohistochemical staining for Iba1 (G, H; scale bar: 20 μm) and GFAP (I, J; scale bar: 50 μm) in the SN ( n =6). * : P <0.05; ** : P <0.01, one-way ANOVA with Newman-Keuls multiple-comparison test. Data are presented as mean±SEM.

Article Snippet: Mouse CXCL1 (GRO-α) and linoleic acid were purchased from MedChemExpress (USA).

Techniques: Enzyme-linked Immunosorbent Assay, Immunohistochemical staining, Staining, Comparison

Journal: Zoological Research

Article Title: Elevated CXCL1 triggers dopaminergic neuronal loss in the substantia nigra of C57BL/6J mice: Evaluation of a novel Parkinsonian mouse model

doi: 10.24272/j.issn.2095-8137.2024.228

Figure Lengend Snippet: CXCL1 induces systemic inflammation in the liver and movement disorders without blood-brain barrier (BBB) injury A: Correlation analysis between serum CXCL1 levels and the number of Iba-1 positive cells in the SN of MPTP-induced chronic model mice (r=0.6610, P <0.05, Pearson correlation test). Two-month-old C57BL/6J male mice received intravenous CXCL1 injections for 2 weeks. B: H&E staining of liver tissue (Scale bar: 100 μm). C: Body weight of mice. D: Fluorescence intensity in mouse brain tissue following intravenous injection of fluorochrome to assess BBB function ( n =6, *** : P <0.001, one-way ANOVA with Newman-Keuls multiple-comparison test). Data are presented as mean±SEM. E–G: Pole test assessing motor behavior in mice ( n =10, 13). H: Representative track visualization images from olfactory discrimination test. I, J: Quantification of accumulated duration in the familiar compartment (I) and total movement distance (J) in the olfactory discrimination test ( n =11). ns: Not significant; * : P <0.05; ** : P <0.01, student’s t- test. Data are presented as mean±SEM.

Article Snippet: Mouse CXCL1 (GRO-α) and linoleic acid were purchased from MedChemExpress (USA).

Techniques: Staining, Fluorescence, Injection, Comparison, Olfactory

Journal: Zoological Research

Article Title: Elevated CXCL1 triggers dopaminergic neuronal loss in the substantia nigra of C57BL/6J mice: Evaluation of a novel Parkinsonian mouse model

doi: 10.24272/j.issn.2095-8137.2024.228

Figure Lengend Snippet: CXCL1 induces microglial activation, iron deposition, α-synuclein aggregation, PPAR inhibition, and dopaminergic neuronal loss across the blood-brain barrier A: Fluorescent imaging of CY3 (red) and TH through immunofluorescence staining (green) of the SN of 2-month-old C57BL/6J male mice following intravenous CY3-CXCL1 administration (20 ng/kg/day for 5 days) (Scale bar: 1 mm, 20 μm). B, C: Immunohistochemical staining of CXCR2 in the SN of mice treated with intravenous CXCL1 for 2 weeks (Scale bar: 100 μm). D, E: Immunohistochemical staining of Iba1 in the SN (Scale bar: 50 μm). F, G: Perls’-DAB staining of iron in the SN (Scale bar: 50 μm). H, I: Immunohistochemical staining of fila-α-syn in the SN (Scale bar: 20 μm). J, K: Immunohistochemical staining of PPARγ in the SN (Scale bars: 50 μm, 10 μm). L, M: Immunohistochemical staining of TH in the SN (Scale bars: 100 μm, 20 μm). n =5, * : P <0.05; ** : P <0.01; *** : P <0.001, student’s t -test. Data are presented as mean±SEM.

Article Snippet: Mouse CXCL1 (GRO-α) and linoleic acid were purchased from MedChemExpress (USA).

Techniques: Activation Assay, Inhibition, Imaging, Immunofluorescence, Staining, Immunohistochemical staining

Journal: Zoological Research

Article Title: Elevated CXCL1 triggers dopaminergic neuronal loss in the substantia nigra of C57BL/6J mice: Evaluation of a novel Parkinsonian mouse model

doi: 10.24272/j.issn.2095-8137.2024.228

Figure Lengend Snippet: Linoleic acid (LA) inhibits movement disorder, neuroinflammation, and dopaminergic neuronal loss induced by CXCL1 A–C: Motor behavior assessment using the pole test in CXCL1-induced mice pre-treated with LA. D–G: Immunohistochemical staining of TH (D, E, scale bars: 100 μm, 20 μm) and Iba1 (F, G, scale bar: 50 μm)-positive cells in the SN ( n =5). * : P <0.05; ** : P <0.01; *** : P <0.001, one-way ANOVA with Newman-Keuls multiple-comparison test. Data are presented as mean±SEM.

Article Snippet: Mouse CXCL1 (GRO-α) and linoleic acid were purchased from MedChemExpress (USA).

Techniques: Immunohistochemical staining, Staining, Comparison

Journal: Zoological Research

Article Title: Elevated CXCL1 triggers dopaminergic neuronal loss in the substantia nigra of C57BL/6J mice: Evaluation of a novel Parkinsonian mouse model

doi: 10.24272/j.issn.2095-8137.2024.228

Figure Lengend Snippet: Graphical abstract A: Flow chart of CXCL1-induced mice with pre-administered linoleic acid. B: Mechanistic diagram.

Article Snippet: Mouse CXCL1 (GRO-α) and linoleic acid were purchased from MedChemExpress (USA).

Techniques:

Journal: Journal of Neuroinflammation

Article Title: An IFNγ/CXCL2 regulatory pathway determines lesion localization during EAE

doi: 10.1186/s12974-018-1237-y

Figure Lengend Snippet: IFNγ suppresses CXCL2 expression in neutrophils and monocytes stimulated with CXCR2 or TLR ligands, respectively. a , b Bone marrow neutrophils were cultured in media alone, or with recombinant CXCL2, CXCL2 plus IL-1β, CXCL1, or G-CSF, in the presence or absence of IFNγ. RNA was extracted 1 h later to measure CXCL2 transcript levels via qPCR. Fold induction of CXCL2 in the stimulated neutrophils over unstimulated controls was calculated as 2 −ΔΔCt , using GAPDH as an internal control for normalization. Each symbol represents a neutrophil culture derived from an individual mouse. ( a n = 8 mice, with data pooled from three experiments. b Representative of two experiments with n = 3 mice each). c Bone marrow monocytes were stimulated with LPS in the presence or absence of IFNγ for 4 h. Intracellular CXCL2 protein expression was assessed by flow cytometry (Representative of three experiments with n = 3 mice each)

Article Snippet: For immunofluorescent histology, primary antibodies included rabbit α-glial fibrillary acidic protein (GFAP) (Gibco), rat α-mouse CD45 (IBL-5/15, Millipore), goat α-mouse CXCL2 (R&D Systems), goat α-mouse CXCL1 (R&D Systems), rat α-mouse Ly6G (IA8, BD Biosciences), and hamster α-mouse CD3ε (BD Biosciences).

Techniques: Expressing, Cell Culture, Recombinant, Control, Derivative Assay, Flow Cytometry

Journal: Journal of Neuroinflammation

Article Title: An IFNγ/CXCL2 regulatory pathway determines lesion localization during EAE

doi: 10.1186/s12974-018-1237-y

Figure Lengend Snippet: CXCL1 is expressed by CNS resident cells during EAE. a CXCL1 (red) was detected in the choroid plexus epithelium in brainstem sections of IFNγRKO mice at the onset of aEAE (clinical score 1), and in astrocytes in spinal cord sections of WT mice at the onset of cEAE (clinical score 2), via immunofluorescent histology. Sections were co-stained with antibodies specific for CD45 (green) and GFAP (white), and counterstained with DAPI (blue). b Choroid plexus samples were obtained at the onset of cEAE or aEAE, or from naïve IFNγRKO mice. CXCL1 transcript was quantified by qPCR and normalized to GAPDH ( n = 4–6/group from two experiments)

Article Snippet: For immunofluorescent histology, primary antibodies included rabbit α-glial fibrillary acidic protein (GFAP) (Gibco), rat α-mouse CD45 (IBL-5/15, Millipore), goat α-mouse CXCL2 (R&D Systems), goat α-mouse CXCL1 (R&D Systems), rat α-mouse Ly6G (IA8, BD Biosciences), and hamster α-mouse CD3ε (BD Biosciences).

Techniques: Staining